| 產(chǎn)品編號 | bs-1293R |
| 英文名稱 | GABBR2 Rabbit pAb |
| 中文名稱 | G氨基丁酸B型受體2抗體 |
| 別 名 | GABR2_HUMAN; gamma-aminobutyric acid type B receptor subunit 2; GPR51; GPRC3B; GABA-B receptor 2; GABA B Receptor 2; GABA-B-R2; GABA-BR2; GABABR2; Gb2; G-protein coupled receptor 51; HG20; |
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Specific References (2) | bs-1293R has been referenced in 2 publications.
[IF=6.792] Zhao D et al. PCB52 exposure alters the neurotransmission ligand-receptors in male offspring and contributes to sex-specific neurodevelopmental toxicity. Environ Pollut.2020 Sep;264:114715. WB ; Rat.
[IF=5.62] Dan Dong. et al. Dapagliflozin inhibits the activity of lateral habenula to alleviate diabetes mellitus-induced depressive-like behavior. EXP NEUROL. 2023 Aug;366:114448 WB ; Rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 通道蛋白 細(xì)胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 103 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABBR2: 501-650/941 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010] Function: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Subunit: Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with ATF4 via its C-terminal region. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. Tissue Specificity: Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. Similarity: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. SWISS: O75899 Gene ID: 9568 Database links: Entrez Gene: 9568 Human Entrez Gene: 242425 Mouse SwissProt: O75899 Human SwissProt: Q80T41 Mouse g-氨基丁酸(GABA)是哺乳動物中樞神經(jīng)系統(tǒng)中重要的抑制性神經(jīng)遞質(zhì)，在體內(nèi)通過作用于離子通道型的GABAA、GABAC受體及代謝型的GABAB受體而發(fā)揮生理功能。 GABAB 受體存在于神經(jīng)元的突觸前及突觸后部位，介導(dǎo)慢的抑制性效應(yīng)，在腦內(nèi)參與許多重要的生理活動和病理變化，包括認(rèn)知損害、癲癇、痙攣及藥物成癮等。 GABAB2在與GABAB1形成二聚體后可激活G蛋白，但它沒有結(jié)合GABA的能力；GABAB2還可增強(qiáng)激動劑與GABAB1的親和力，并且通過與GABAB1形成二聚體結(jié)構(gòu)而屏蔽GABAB1的胞內(nèi)滯留信號。 |
| 產(chǎn)品圖片 |
Sample:
Brain(Mouse) Lysate at 40 ug
Primary: Anti-GABA B Receptor 2 (bs-1293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103 kD
Observed band size: 125 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-GABA B Receptor 2 (bs-1293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 103 kD
Observed band size: 125 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABA B Receptor 2) Polyclonal Antibody, Unconjugated (bs-1293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABA B Receptor 2) Polyclonal Antibody, Unconjugated (bs-1293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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