| 產(chǎn)品編號 | bs-1610R-PE |
| 英文名稱 | Rabbit Anti-CK7/PE Conjugated antibody |
| 中文名稱 | PE標記的細胞角蛋白7抗體 |
| 別 名 | Cytokeratin 7; CK 7; K7; Keratin 7; Keratin,type II cytoskeletal 7; KRT7; SCL; K2C7_HUMAN. |
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Journal
PMID
IF
Application
[IF=6.774] IF ; rabbit.
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| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 信號轉導 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, ) |
| 產(chǎn)品應用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 54kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human CK7 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Function: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Subunit: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. Post-translational modifications: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Belongs to the intermediate filament family. Database links: Entrez Gene: 3855 Human Omim: 148059 Human SwissProt: P08729 Human Unigene: 411501 Human Unigene: 670221 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 結構蛋白（Structural Proteins） CK-7是一種 54KDa 的中間絲蛋白，存在于大多數(shù)正常組織的腺上皮和移行上皮細胞中。 該抗體與多種良/惡性上皮性腫瘤反應。腺癌中的卵巢、乳腺、肺的腺癌呈陽性反應，而胃腸道的腺癌陰性。移行細胞腫瘤、前列腺癌也呈陽性反應。通常認為 CK7是腺癌和移行上皮細胞癌的比較特異性的標志。 |
| 產(chǎn)品圖片 |
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CK7 antibody (bs-1610R-PE)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
