| 產(chǎn)品編號 | bs-1556R-AP |
| 英文名稱 | Rabbit Anti-Profilin 1/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標(biāo)記的前纖維蛋白1抗體 |
| 別 名 | Actin binding protein; PFN 1; Pfn; PFN1; Profilin I; Profilin1; ProfilinI; PROF1_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞周期蛋白 細(xì)胞分化 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Profilin 1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Profilin 1 is a ubiquitous actin monomer-binding protein involved in actin polymerization in response to extracellular signals. Three profilin genes have been identified: profilin 1, 2, and 3. Profilin 1 is the most ubiquitous and abundant, and is highly expressed throughout development and adulthood inmost tissues including brain. Profilin 2 is the neuronal specific isoform and profilin 3 is a testis specific isoform. Profilins of eukaryotic cells are small (12-15 kDa) cytoplasmic proteins that bind to actin monomers, polyphosphoinositides and polymers of L-proline. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype. Function: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. Subunit: Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with VASP. Interacts with HTT. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Expressed in epididymis (at protein level). Post-translational modifications: Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation. DISEASE: Amyotrophic lateral sclerosis 18 (ALS18) [MIM:614808]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the profilin family. Database links: Entrez Gene: 5216 Human Entrez Gene: 18643 Mouse Omim: 176610 Human SwissProt: P07737 Human SwissProt: P62962 Mouse Unigene: 494691 Human Unigene: 2647 Mouse Unigene: 1152 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 前纖維蛋白(profilin)是一種低分子量的肌動(dòng)蛋白結(jié)合蛋白, 前纖維蛋白除了能與肌動(dòng)蛋白結(jié)合之外,在分子間相互作用的復(fù)雜網(wǎng)絡(luò)中還起到一個(gè)控制中心的作用。 |
