| 產(chǎn)品編號 | bs-6874R |
| 英文名稱 | CAT Rabbit pAb |
| 中文名稱 | 過氧化氫酶抗體 |
| 別 名 | CATA_HUMAN; Catalase; EC:1.11.1.6. |
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Specific References (9) | bs-6874R has been referenced in 9 publications.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 發(fā)育生物學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Catalase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Subunit: Homotetramer. Subcellular Location: Peroxisome. Post-translational modifications: The N-terminus is blocked. DISEASE: Defects in CAT are the cause of acatalasemia (ACATLAS) [MIM:614097]. A metabolic disorder characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. Similarity: Belongs to the catalase family. SWISS: P24270 Gene ID: 12359 Database links: Entrez Gene: 847 Human Entrez Gene: 12359 Mouse Omim: 115500 Human SwissProt: P04040 Human SwissProt: P24270 Mouse Unigene: 502302 Human Unigene: 4215 Mouse Unigene: 3001 Rat 過氧化氫酶是催化過氧化氫分解成氧和水的酶，存在于細胞的過氧化物體內(nèi)。過氧化氫酶是過氧化物酶體的標志酶, 約占過氧化物酶體酶總量的40%。過氧化氫酶存在于所有已知的動物的各個組織中，特別在肝臟中以高濃度存在。過氧化氫酶在食品工業(yè)中被用于除去用于制造奶酪的牛奶中的過氧化氫。過氧化氫酶也被用于食品包裝，防止食物被氧化。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Placenta tissue lysates
Lane 3: Rat Heart tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-CAT (bs-6874) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated(bs-6874R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-Catalase antibody (bs-6874R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
