| 產(chǎn)品編號 | bsm-33192M |
| 英文名稱 | GAP43 Mouse mAb |
| 中文名稱 | 神經(jīng)生長相關(guān)蛋白43單克隆抗體 |
| 別 名 | Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071. |
| 研究領(lǐng)域 | 腫瘤 免疫學 神經(jīng)生物學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6F11 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GAP43 Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Subunit: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes. Post-translational modifications: Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. Similarity: Belongs to the neuromodulin family. Contains 1 IQ domain. SWISS: P17677 Gene ID: 2596 Database links: Entrez Gene: 2596 Human Entrez Gene: 14432 Mouse GenBank: NP_002036 Human Omim: 162060 Human SwissProt: P17677 Human SwissProt: P06837 Mouse Unigene: 134974 Human Unigene: 1222 Mouse Unigene: 10928 Rat 神經(jīng)生物學相關(guān)蛋白（Neurobiology）；神經(jīng)標志物 GAP43 （Growth associated protein-43）又稱作neuromodulin，是一個軸突膜蛋白，是一種神經(jīng)特異性的蛋白質(zhì)，參與神經(jīng)細胞外生長及突觸發(fā)育形成和神經(jīng)細胞再生。在神經(jīng)元發(fā)育和再生過程中以高水平表達。能調(diào)解軸突延伸作用，改變細胞形態(tài)。作為細胞內(nèi)信號，可大大增強與G蛋白偶聯(lián)的受體轉(zhuǎn)運作用。 神經(jīng)生長相關(guān)蛋白-GAP-43和神經(jīng)細胞黏附因子(neural cell adhesion molecule,NCAM)與突觸可塑性密切相關(guān).GAP-43是一種神經(jīng)細胞膜上的特異性磷蛋白,在神經(jīng)發(fā)育和再生過程中呈現(xiàn)高表達,被作為突觸生長的標志物,有稱脊髓生長相關(guān)蛋白 GAP-43與CaM結(jié)合,參與G蛋白相互作用,神經(jīng)遞質(zhì)的釋放,作用于胞吞/胞吐過程,通過小囊溶合或誘導生長錐和突觸前末端的胞吞促進膜擴展,與海馬長時程增強密切相關(guān). 神經(jīng)細胞黏附因子是細胞表面糖蛋白大家族的成員之一,促進軸突生長,對長時記憶的保持有重要影響,同時,GAP-43對其具調(diào)節(jié)作用，廣泛而深入地研究GAP-43意義深遠。 |
| 產(chǎn)品圖片 |
Sample:
BV2 (Mouse) Lysate at 40 ug
VSC4.1(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43-46 kD
Observed band size: 43 kD
Sample:
Spinal cord(Mouse) Lysate at 40 ug
Spinal cord(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43-46 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GAP43 ) Monoclonal Antibody, Unconjugated (bsm-33192M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GAP43 ) Monoclonal Antibody, Unconjugated (bsm-33192M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GAP43 ) Monoclonal Antibody, Unconjugated (bsm-33192M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (ascites of bsm-33192M 5F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bsm-33192M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GAP43) monoclonal Antibody, unconjugated (bsm-33192M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-40296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
